clone method 克隆方式;复制方法
- Gene cloning comprise two types of cloning methods: positional cloning and phenotype cloning.
基因克隆一般分为定位克隆和表型克隆。 - Methods Molecular cloning, sequencing and bioinformatics techniques were used to identify the difference between the new HLA allele and other known alleles.
方法采用基因克隆、测序及生物信息学技术,分析HLA新等位基因与HLA已知基因序列的差异。 - However the former cloning methods such as map-base cloning or transposon tagging which based on forward genetics can only cloned one or several genes at the same time.
以往的基因克隆方法如图位克隆法、转座子标签法等大都是建立在正向遗传学的基础上,这些方法只适用于一个或少数基因,应该说是零敲碎打的策略。
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