This is then coated with agarose, a nutrient gel that bacteria will grow on. 在印章上图一层琼脂糖,细菌就能够在这种营养凝胶体上生长。
Boil1 g Agarose in100 ml Gel running buffer( microwave:45 sec, swirl,20 sec, swirl,20 sec). Let cool for10 min. 将1克的琼脂洋菜粉末加入100毫升的胶体电泳缓冲液并煮沸(波炉:45秒,晃几下,20秒,晃几下,20秒)等10分钟待其降温。
He pipettes solutions of bacteria onto the agarose, which sucks out the water, leaving a solid layer of bacteria. 然后他用吸液管将细菌溶液滴到琼脂糖上,在琼脂糖吸干了溶液中的水分后,印章表面就会形成一层固体细菌。